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Image Search Results
Journal: Bio-protocol
Article Title: Kinase Mobility Shift Assay (KiMSA) for Assessing Protein Kinase A Activity
doi: 10.21769/BioProtoc.5366
Figure Lengend Snippet: (A) Representative agarose gel electrophoresis image obtained after KiMSA. The left lane contains non-phosphorylated Kemptide-FITC, and the right lane contains Kemptide-FITC incubated with recPKA-C at 37 °C for 30 min. (B) Excitation (blue, left, λem = 550 nm) and Emission (green, right, λex = 460 nm) spectra of Kemptide-FITC (shaded area) and phosphorylated Kemptide-FITC (p-Kemp-FITC) (dashed lines). No spectral shifts were observed between phosphorylated and non-phosphorylated Kemptide-FITC. Excitation and emission maxima are 489 nm and 516 nm, respectively.
Article Snippet:
Techniques: Agarose Gel Electrophoresis, Incubation